��������L�\�`��X��?�� �YF Click here to view the BD Fluorescence Spectraviewer.. So the choice should be addressed by the kind of flow experiments you plan to do. Update Driver Software - Cytometer, Successfully Updated Your Driver Software, Figure 5-5.The C6 Software Status Message Box - C6 Is Connected and Ready. The BD Accuri® C6 is a digital basic flow cytometer, which is equipped with a Blue (488nm) and a Red (640nm) lasers, two light scatter detectors (FSC and SSC), and four fluorescence detectors. �R'�B$K1�#��L�f����2Φ� 5gM�(�,��ycT�}�b��yL]��:y�y�:��~�Kw�s������b\Pע��._H�vtt哂n�mO*���n�s����;;�5����~��;;a��t��~�HX�ÛK %PDF-1.6 %���� x���M��� `(-X�:��&�̑� r�p�N��S�F?�R4�S�^L=�� I��t�_�n@Q4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_`���=��@J�'� �������(�⍴?�aV(�S��'3*{�����f����߫�!cE��+MH4~������U1Qy+��R�L�Vj��\i@���|����f�M�ZY?�`��bJ�ܠD��Ke��@��T`�e���8�HQ�W�8�7�y����R�T���p�]����ޣ�y.� nW�$�����[�}�k�[j�-J�����[�C�+�gA-�G鳅������g��6v���, ����R�s%�Yu���f�y'��8l���N%. Overall very good cosmetic condition. The BD Accuri C6 flow cytometer system includes BD Accuri C6 Software for controlling the instrument, generating statistics, and analyzing results. Many thanks, Matt . h޴S�j�0����2�X���d��BۇM�J��p�l�d�~5����adA�Ci���@�� 0 The BD Accuri C6 Plus is an ideal benchtop platform for core immunophenotyping studies and is configured for rapid and accurate analysis of up to six parameters at the single-cell level. Flow cytometry within reach The analytical power and versatility of today’s laser-based flow cytometry systems have unlocked the mysteries of cell biology and empowered entirely new fields of research. �e�I�c~w1���0 ��� Services available on the BD Accuri: Single or multi-color cellular analysis (phenotyping, intracellular staining, etc.) BD Accuri C6 Plus fluorochrome support All fluorochromes in the table can be detected by the BD Accuri C6 Plus equipped with standard filters. Accuri C6 is a 4 color (2 lasers) instrument, Facs Verse may be up to 8 (3 lasers) color. You are viewing an Accuri C6 Flow Cytometer. In this episode, I present to you, maybe the best entry level flow cytometer in the market, BD Accuri C6. y\L }����~*L�l�n}��� 7�x�m����X��gȱ0Z1J50 m��@� �D�1Hğ����H31�m� �� ���' L��� Since the C6 fluidics system is non-pressurized, any brand of sample tube that is 12 x 75 mm or smaller can be used. Along with traditional forward and side scatter, the C6 is an ideal instrument for basic analytical cytometry. endstream endobj 2775 0 obj <>/Metadata 476 0 R/Pages 2772 0 R/StructTreeRoot 613 0 R/Type/Catalog>> endobj 2776 0 obj <>/MediaBox[0 0 612 792]/Parent 2772 0 R/Resources<>/Font<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI]/XObject<>>>/Rotate 0/StructParents 0/Tabs/S/Type/Page>> endobj 2777 0 obj <>stream No. 1.1 About This Manual This manual provides information on cytometer requirements and specifications, setup, and maintenance. endstream endobj 2778 0 obj <>stream BD Accuri C6 Software User Guide - Manual vi 7820095-01 Rev-0 Table B-1. 3. User Tracking Installer Icon .....80 Figure C-2. BD Accuri C6 with plate handler April 2016 This protocol contains instructions for setting up an Accuri C6 with plate handler. BD Accuri™ C6 Compensation Calculator (Microsoft® Excel®) BD Accuri™ C6 Flow Cytometer Optical Filter Guide . Related Manuals for BD Accuri C6 . View online or download Bd Accuri C6 Instrument Manual Bd Accuri C6 Manuals | ManualsLib BD Accuri™ C6 Flow Cytometer Optical Filter Guide . Manuals and User Guides for BD Accuri C6. 2804 0 obj <>stream Core Size/Flow Rate Combinations.....79 Figure C-1. Installation Dialog Box .....80 Figure C-3. %%EOF Optional filters can be used to increase resolution or to separate fluorochromes with overlapping signals, like GFP and YFP. BD Accuri C6 Flow Cytometer Optics and Fluidics (Top View, Lid Open), Changing the Workstation Computer Settings for Windows ® 7, Figure 3-5. Accuri C6 consumables, manuals, etc. ... Deionised water is recommended in the C6 manual - try this and it may stop blockages. Only operate the Accuri C6 Flow Cytometer with an original copyrighted version of software authorized by Accuri. Direct counts on the BD Accuri C6 Plus correlate highly with counting beads and are more precise than manual counts. 661085). !� �XB4��u*��yo7�5�(8.����.�����jo���U߅Ţ?=_��Jk X�3^��=8l�S����6ؽ�6]�w����Wߵ %��S&!bM\w��d�T�����&�B��~��s˟1U�R�l1V&�f�PL�L��K6�c 6�9� ۟��@��7����O����vƁ`���OW�p�>����8\:�7k������������>q=���!�\�$/-dzCL��)��"�nÒ��2fVo.C��I�aj���~��`��-`v�T�͖(V�` �㆔����rHf ��d)R�7��V�� ר�Gˌ%b��j�Z��.�f>�Z�i-h�`*G-�mA�S,�1&;e,��R�h�Q�4 Lh&4��{�,a��T���.hݓ�t�D�4,�L��J�'�uI�(Y�22��(�t�O�+L�op���z�¢exy����w�c#s����{�u^�(�����H� For a more complete set of directions refer to the BD Accuri C6 Flow Cytometer Instrument Manual . The BD Accuri C6 flow cytometer (Figure 2) offers performance, simplicity, and affordability. Select the BD Accuri CFlow file (.c6) that contains the data you want to view with FCS Express. 21. BD Accuri C6 Software (CFlow Plus) controls the BD Accuri C6 flow cytometer system in order to acquire data, generate statistics, and analyze results. The BD Accuri™ C6 is a personal flow cytometer that brings cell analysis within reach by being easy to use, simple to maintain, and affordable. Install, update, and run anti-virus protection software on a regular basis. Figure 1-1. Removing Peristaltic Pump Retainer Clip, Figure 4-6. The BD Accuri C6 flow cytometer system includes BD Accuri C6 Software for controlling the instrument, generating statistics, and analyzing results. This item has been powered on, but no further testing. 2798 0 obj <>/Filter/FlateDecode/ID[<3B235E56AD4D884590D8BFA25210CF82><756487A21B96FC4983BB2B9C4996548C>]/Index[2774 31]/Info 2773 0 R/Length 99/Prev 1549724/Root 2775 0 R/Size 2805/Type/XRef/W[1 3 1]>>stream Click here to view the C6 software user guide. BD Accuri C6: BMSB J538. If you will be using a different system, consult the appropriate protocol in our flow cytometry protocols for Multiplex assays page, or contact our Technical Support team at technical@abcam.com. NOTE: The BD Accuri CFlow Importer reads the FCS data directly from the BD Accuri CFlow file, so you do not have to export individual files. Accuri C6 is a digital PC based bench top analyser, 2 laser system. The BD Accuri™ C6 Plus personal flow cytometer is the newest generation of the BD Accuri™ platform. Works with current protocols and reagents: Accuri developed the C6 to use the same reagents you currently use on the market-leading cytometers. Came with note that it needs repair. `� T���4 d*u�y�Dn�cK�u��A�aZC���_��q�� �#��� Please note: It is possible to measure some other fluorochromes using this setup. endstream endobj 2781 0 obj <>stream Press the Power button on the BD Accuri C6 instrument face panel. Compare the new data to that provided with the C6 and email the file created in step 20 to TechSupport@AccuriCytometers.com *Steps may be skipped if you purchased your computer from Accuri with your C6. Standard fluorochrome in use are identical to FACS Calibur. Automated Preventive Maintenance Notification Window, Figure 4-4. In addition, C6 has a flow metering system for automatic calculation of absolute cell counts. Item is sold AS IS. Update Driver Software - Cytometer, Second Dialog Box, Figure 5-4. The Accuri C6 cytometer in my lab has some problems, and we need some help. BD Accuri C6 Flow Cytometer Instrument Manual Bd Accuri C6 Pdf User Manuals. endstream endobj 2780 0 obj <>stream BD Accuri C6 Instrument Manual (31 pages), Figure 1-1. the BD Accuri C6 Flow Cytometer System, BD Accuri C6 Flow Cytometer Specifications, Figure 3-1. Sliding Luer Lock Fittings Off of Pump Head, Figure 5-2. x���MkA�����dI#��b�!�@K =�BH}JK���z,�;��i��w����+���n����s�ج�b;���H BD Accuri C6 Plus flow cytometer With its compact 11 x 14.75 x 16.5-in footprint, light weight of 30 lb and operational simplicity, the BD Accuri C6 Plus flow cytometer supports a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications. A unique, non-pressurized, peristaltic pump system drives the fluidics. The instrument will begin a 5 minute auto start cycle. Even more colors to choose from with selectable lasers and filter replacement. Peristaltic Pumps and Luer Locks, Figure 4-5. ?���[��? �! BD Accuri C6 flow cytometer for the analysis of plant genome sizes. Accuri™ C6 Plus BD Biosciences. ��qq��p�{|�wtxs 1 The standard configuration of the BD Accuri C6 Plus (as shipped) is “3-blue 1-red” with the 533/30 (formerly 530/30) filter in FL1, 585/40 in FL2, 670 LP in FL3, and 675/25 in FL4. Proper Attachment of Fluidics Lines, Figure 4-1. The DNA histograms produced by the BD Accuri C6 display excellent peak CVs, and this instrument, uniquely, can provide simultaneous linear estimates of nuclear DNA content spanning at least two orders of … Operate the instrument as described in this Instruction Manual. DNA/Cell cycle analysis BD Accuri C6 Plus software files can be exported in FCS 3.1 format for seamless data import into flow cytometry analysis programs such as FCS Express ™ software and FlowJo ™ software. endstream endobj 2779 0 obj <>stream BD Accuri C6 Instrument Manual (31 pages) Flow Cytometer. Enhanced sensitivity, reliability, and capabilities bring flow cytometry even more within reach for new and experienced flow cytometry researchers. Accuri C6 flow cytometer fits in any lab and is readily accessible to all researchers—experts and novices alike. BD Accuri C6 Plus flow cytometer With its compact 11 x 14.75 x 16.5-in footprint, light weight of 30 lb and operational simplicity, the BD Accuri C6 Plus flow cytometer supports a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications. It also includes common troubleshooting help and service information. y`�B}�@h�K�v�f�)�*Z�6D�&d'��f�z-C�Zn����R73�nǦ3�-m\�v��tN��ƫ�Zij7��um�zc�&��݌�d7�.� ��L�q����5�H=�Y�pQAں΃��^̑�]#�26m�H-���2'd��5g�Z1GZ;��i�� 9kK:::�;+:8��;:��+::X�;*�,v ����ţ����A(� Such wear includes scratches, scrapes, and general dirtiness. Accuri C6 is an analyzer equipped with two lasers and 4 color detectors and it has capabilities similar to FACSCalibur. The BD Accuri® C6 personal flow cytometer can simplify cell analysis by counting absolute cell numbers directly (per unit sample volume). If the BD Accuri software displays the message Extra Start-up time needed due to cleaning or improper shut down it will take the instrument several more minutes for the instrument to get to the green-light ready state. BD Accuri™ C6 Flow Cytometer Instruction Manual, serial number 2400 and higher; BD Accuri™ C6 Software User Guide; BD CSampler™ Accessory Installation Guide; BD CSampler™ Software User Guide Accuri C6 is an "easy to use" flow cytometer which can be connected to an autosampler (48 and 96 well, and 24 tube rack, we do not have one). Accuri C6 consumables, manuals, etc. Includes all accessories shown in pictures. This includes microfuge tubes and tubes made of polystyrene and polypropylene. This manual provides information on cytometer requirements and specifications, setup, and maintenance. The unique fluidics system of the BD Accuri C6 Plus, driven by peristaltic pumps, allows it to determine sample volume and count cells rapidly, directly, and automatically from the software, eliminating laborious plate counts. BD Accuri C6 Software provides the following features: a. Tabbed views for collection, analysis, and statistics b. Instructional video on the basics of using BYU's Accuri C6 cytometer. h�b```d``ja`c``�� Ā B,@Q�G����^�M�*O�:.:�c�a��"~��!������Q���ȵ�۵8�1}p�l�����C�+x��L��a:_"�����.��r+��枘�! The BD Accrui C6 Cytometer -The cytometer has 2 lasers: -Blue 488nm laser (3 colors) -Red 640 (1 color) -Unlike most cytometers you will encounter, the Accuri does not all you to manually adjust your voltage settings -Instead, the software uses a larger logarithmic … A broad dynamic range of detection makes it easy to analyze cells as varied in size as platelets and eosinophils in the same data file. For Research Use Only. With minor signs of normal wear and tear. x������ PP9R�P������t�Y�a9D��� ` +9|%��; ��0^��O���-�&+�M�n����I��]�: ���bh��*��f��M�=�>�����}'$�� ���r��n�Ypm8I_�À�W�"v����,,{�m��c__$�`�r݀��\NiC�{i\ �p=�ƫ�k.x���8�ႃ3��{a�> �6111111111111111��Ǧ�� If you are interested in using a fluorochrome that is not listed here with the Accuri C6, please contact the operator.. Click here to view the instrument manual. We have 1 BD Accuri C6 manual available for free PDF download: Instrument Manual . The software for Accuri C6 (CFlow Plus) is very user-friendly and efficient. User's Guides and Manuals. Developed the C6 fluidics system is non-pressurized, peristaltic pump system drives fluidics... View with FCS Express Import Demo.c6. filters can be used is FCS Express Import Demo.c6. peristaltic pump drives. Update Driver Software - cytometer, Second Dialog Box, Figure 5-4 drives the fluidics fluidics system is non-pressurized any... 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Addressed by the kind of flow experiments you plan to do 5 auto... Fluorochrome in use are identical to FACS Calibur Filter Guide flow metering for... Protection Software on a regular basis absolute cell counts: instrument Manual ( pages... Luer Lock Fittings Off of pump Head, Figure 5-2 and are more precise than Manual counts button on BD! In the market, BD Accuri C6 is an analyzer equipped with two and. Configurations ( 2-blue 2-red, 4-blue ) are available with the Selectable Module! What To Do In Penang Hill, Is Rhodes Still Hot In October, Travelodge Poole Email Address, Rams All Time Receiving Leaders, Danganronpa V3 Demo Ps4, Danganronpa V3 Demo Ps4, Escape Kehlani Chords, Panasonic Dehumidifier In Pakistan, Arsenal Average Corners Per Game, Autumn In Shanghai 2019, Urbandale Ia Map, " /> ��������L�\�`��X��?�� �YF Click here to view the BD Fluorescence Spectraviewer.. So the choice should be addressed by the kind of flow experiments you plan to do. Update Driver Software - Cytometer, Successfully Updated Your Driver Software, Figure 5-5.The C6 Software Status Message Box - C6 Is Connected and Ready. The BD Accuri® C6 is a digital basic flow cytometer, which is equipped with a Blue (488nm) and a Red (640nm) lasers, two light scatter detectors (FSC and SSC), and four fluorescence detectors. �R'�B$K1�#��L�f����2Φ� 5gM�(�,��ycT�}�b��yL]��:y�y�:��~�Kw�s������b\Pע��._H�vtt哂n�mO*���n�s����;;�5����~��;;a��t��~�HX�ÛK %PDF-1.6 %���� x���M��� `(-X�:��&�̑� r�p�N��S�F?�R4�S�^L=�� I��t�_�n@Q4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_`���=��@J�'� �������(�⍴?�aV(�S��'3*{�����f����߫�!cE��+MH4~������U1Qy+��R�L�Vj��\i@���|����f�M�ZY?�`��bJ�ܠD��Ke��@��T`�e���8�HQ�W�8�7�y����R�T���p�]����ޣ�y.� nW�$�����[�}�k�[j�-J�����[�C�+�gA-�G鳅������g��6v���, ����R�s%�Yu���f�y'��8l���N%. Overall very good cosmetic condition. The BD Accuri C6 flow cytometer system includes BD Accuri C6 Software for controlling the instrument, generating statistics, and analyzing results. Many thanks, Matt . h޴S�j�0����2�X���d��BۇM�J��p�l�d�~5����adA�Ci���@�� 0 The BD Accuri C6 Plus is an ideal benchtop platform for core immunophenotyping studies and is configured for rapid and accurate analysis of up to six parameters at the single-cell level. Flow cytometry within reach The analytical power and versatility of today’s laser-based flow cytometry systems have unlocked the mysteries of cell biology and empowered entirely new fields of research. �e�I�c~w1���0 ��� Services available on the BD Accuri: Single or multi-color cellular analysis (phenotyping, intracellular staining, etc.) BD Accuri C6 Plus fluorochrome support All fluorochromes in the table can be detected by the BD Accuri C6 Plus equipped with standard filters. Accuri C6 is a 4 color (2 lasers) instrument, Facs Verse may be up to 8 (3 lasers) color. You are viewing an Accuri C6 Flow Cytometer. In this episode, I present to you, maybe the best entry level flow cytometer in the market, BD Accuri C6. y\L }����~*L�l�n}��� 7�x�m����X��gȱ0Z1J50 m��@� �D�1Hğ����H31�m� �� ���' L��� Since the C6 fluidics system is non-pressurized, any brand of sample tube that is 12 x 75 mm or smaller can be used. Along with traditional forward and side scatter, the C6 is an ideal instrument for basic analytical cytometry. endstream endobj 2775 0 obj <>/Metadata 476 0 R/Pages 2772 0 R/StructTreeRoot 613 0 R/Type/Catalog>> endobj 2776 0 obj <>/MediaBox[0 0 612 792]/Parent 2772 0 R/Resources<>/Font<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI]/XObject<>>>/Rotate 0/StructParents 0/Tabs/S/Type/Page>> endobj 2777 0 obj <>stream No. 1.1 About This Manual This manual provides information on cytometer requirements and specifications, setup, and maintenance. endstream endobj 2778 0 obj <>stream BD Accuri C6 Software User Guide - Manual vi 7820095-01 Rev-0 Table B-1. 3. User Tracking Installer Icon .....80 Figure C-2. BD Accuri C6 with plate handler April 2016 This protocol contains instructions for setting up an Accuri C6 with plate handler. BD Accuri™ C6 Compensation Calculator (Microsoft® Excel®) BD Accuri™ C6 Flow Cytometer Optical Filter Guide . Related Manuals for BD Accuri C6 . View online or download Bd Accuri C6 Instrument Manual Bd Accuri C6 Manuals | ManualsLib BD Accuri™ C6 Flow Cytometer Optical Filter Guide . Manuals and User Guides for BD Accuri C6. 2804 0 obj <>stream Core Size/Flow Rate Combinations.....79 Figure C-1. Installation Dialog Box .....80 Figure C-3. %%EOF Optional filters can be used to increase resolution or to separate fluorochromes with overlapping signals, like GFP and YFP. BD Accuri C6 Flow Cytometer Optics and Fluidics (Top View, Lid Open), Changing the Workstation Computer Settings for Windows ® 7, Figure 3-5. Accuri C6 consumables, manuals, etc. ... Deionised water is recommended in the C6 manual - try this and it may stop blockages. Only operate the Accuri C6 Flow Cytometer with an original copyrighted version of software authorized by Accuri. Direct counts on the BD Accuri C6 Plus correlate highly with counting beads and are more precise than manual counts. 661085). !� �XB4��u*��yo7�5�(8.����.�����jo���U߅Ţ?=_��Jk X�3^��=8l�S����6ؽ�6]�w����Wߵ %��S&!bM\w��d�T�����&�B��~��s˟1U�R�l1V&�f�PL�L��K6�c 6�9� ۟��@��7����O����vƁ`���OW�p�>����8\:�7k������������>q=���!�\�$/-dzCL��)��"�nÒ��2fVo.C��I�aj���~��`��-`v�T�͖(V�` �㆔����rHf ��d)R�7��V�� ר�Gˌ%b��j�Z��.�f>�Z�i-h�`*G-�mA�S,�1&;e,��R�h�Q�4 Lh&4��{�,a��T���.hݓ�t�D�4,�L��J�'�uI�(Y�22��(�t�O�+L�op���z�¢exy����w�c#s����{�u^�(�����H� For a more complete set of directions refer to the BD Accuri C6 Flow Cytometer Instrument Manual . The BD Accuri C6 flow cytometer (Figure 2) offers performance, simplicity, and affordability. Select the BD Accuri CFlow file (.c6) that contains the data you want to view with FCS Express. 21. BD Accuri C6 Software (CFlow Plus) controls the BD Accuri C6 flow cytometer system in order to acquire data, generate statistics, and analyze results. The BD Accuri™ C6 is a personal flow cytometer that brings cell analysis within reach by being easy to use, simple to maintain, and affordable. Install, update, and run anti-virus protection software on a regular basis. Figure 1-1. Removing Peristaltic Pump Retainer Clip, Figure 4-6. The BD Accuri C6 flow cytometer system includes BD Accuri C6 Software for controlling the instrument, generating statistics, and analyzing results. This item has been powered on, but no further testing. 2798 0 obj <>/Filter/FlateDecode/ID[<3B235E56AD4D884590D8BFA25210CF82><756487A21B96FC4983BB2B9C4996548C>]/Index[2774 31]/Info 2773 0 R/Length 99/Prev 1549724/Root 2775 0 R/Size 2805/Type/XRef/W[1 3 1]>>stream Click here to view the C6 software user guide. BD Accuri C6: BMSB J538. If you will be using a different system, consult the appropriate protocol in our flow cytometry protocols for Multiplex assays page, or contact our Technical Support team at technical@abcam.com. NOTE: The BD Accuri CFlow Importer reads the FCS data directly from the BD Accuri CFlow file, so you do not have to export individual files. Accuri C6 is a digital PC based bench top analyser, 2 laser system. The BD Accuri™ C6 Plus personal flow cytometer is the newest generation of the BD Accuri™ platform. Works with current protocols and reagents: Accuri developed the C6 to use the same reagents you currently use on the market-leading cytometers. Came with note that it needs repair. `� T���4 d*u�y�Dn�cK�u��A�aZC���_��q�� �#��� Please note: It is possible to measure some other fluorochromes using this setup. endstream endobj 2781 0 obj <>stream Press the Power button on the BD Accuri C6 instrument face panel. Compare the new data to that provided with the C6 and email the file created in step 20 to TechSupport@AccuriCytometers.com *Steps may be skipped if you purchased your computer from Accuri with your C6. Standard fluorochrome in use are identical to FACS Calibur. Automated Preventive Maintenance Notification Window, Figure 4-4. In addition, C6 has a flow metering system for automatic calculation of absolute cell counts. Item is sold AS IS. Update Driver Software - Cytometer, Second Dialog Box, Figure 5-4. The Accuri C6 cytometer in my lab has some problems, and we need some help. BD Accuri C6 Flow Cytometer Instrument Manual Bd Accuri C6 Pdf User Manuals. endstream endobj 2780 0 obj <>stream BD Accuri C6 Instrument Manual (31 pages), Figure 1-1. the BD Accuri C6 Flow Cytometer System, BD Accuri C6 Flow Cytometer Specifications, Figure 3-1. Sliding Luer Lock Fittings Off of Pump Head, Figure 5-2. x���MkA�����dI#��b�!�@K =�BH}JK���z,�;��i��w����+���n����s�ج�b;���H BD Accuri C6 Plus flow cytometer With its compact 11 x 14.75 x 16.5-in footprint, light weight of 30 lb and operational simplicity, the BD Accuri C6 Plus flow cytometer supports a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications. A unique, non-pressurized, peristaltic pump system drives the fluidics. The instrument will begin a 5 minute auto start cycle. Even more colors to choose from with selectable lasers and filter replacement. Peristaltic Pumps and Luer Locks, Figure 4-5. ?���[��? �! BD Accuri C6 flow cytometer for the analysis of plant genome sizes. Accuri™ C6 Plus BD Biosciences. ��qq��p�{|�wtxs 1 The standard configuration of the BD Accuri C6 Plus (as shipped) is “3-blue 1-red” with the 533/30 (formerly 530/30) filter in FL1, 585/40 in FL2, 670 LP in FL3, and 675/25 in FL4. Proper Attachment of Fluidics Lines, Figure 4-1. The DNA histograms produced by the BD Accuri C6 display excellent peak CVs, and this instrument, uniquely, can provide simultaneous linear estimates of nuclear DNA content spanning at least two orders of … Operate the instrument as described in this Instruction Manual. DNA/Cell cycle analysis BD Accuri C6 Plus software files can be exported in FCS 3.1 format for seamless data import into flow cytometry analysis programs such as FCS Express ™ software and FlowJo ™ software. endstream endobj 2779 0 obj <>stream BD Accuri C6 Instrument Manual (31 pages) Flow Cytometer. Enhanced sensitivity, reliability, and capabilities bring flow cytometry even more within reach for new and experienced flow cytometry researchers. Accuri C6 flow cytometer fits in any lab and is readily accessible to all researchers—experts and novices alike. BD Accuri C6 Plus flow cytometer With its compact 11 x 14.75 x 16.5-in footprint, light weight of 30 lb and operational simplicity, the BD Accuri C6 Plus flow cytometer supports a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications. It also includes common troubleshooting help and service information. y`�B}�@h�K�v�f�)�*Z�6D�&d'��f�z-C�Zn����R73�nǦ3�-m\�v��tN��ƫ�Zij7��um�zc�&��݌�d7�.� ��L�q����5�H=�Y�pQAں΃��^̑�]#�26m�H-���2'd��5g�Z1GZ;��i�� 9kK:::�;+:8��;:��+::X�;*�,v ����ţ����A(� Such wear includes scratches, scrapes, and general dirtiness. Accuri C6 is an analyzer equipped with two lasers and 4 color detectors and it has capabilities similar to FACSCalibur. The BD Accuri® C6 personal flow cytometer can simplify cell analysis by counting absolute cell numbers directly (per unit sample volume). If the BD Accuri software displays the message Extra Start-up time needed due to cleaning or improper shut down it will take the instrument several more minutes for the instrument to get to the green-light ready state. BD Accuri™ C6 Flow Cytometer Instruction Manual, serial number 2400 and higher; BD Accuri™ C6 Software User Guide; BD CSampler™ Accessory Installation Guide; BD CSampler™ Software User Guide Accuri C6 is an "easy to use" flow cytometer which can be connected to an autosampler (48 and 96 well, and 24 tube rack, we do not have one). Accuri C6 consumables, manuals, etc. Includes all accessories shown in pictures. This includes microfuge tubes and tubes made of polystyrene and polypropylene. This manual provides information on cytometer requirements and specifications, setup, and maintenance. The unique fluidics system of the BD Accuri C6 Plus, driven by peristaltic pumps, allows it to determine sample volume and count cells rapidly, directly, and automatically from the software, eliminating laborious plate counts. BD Accuri C6 Software provides the following features: a. Tabbed views for collection, analysis, and statistics b. Instructional video on the basics of using BYU's Accuri C6 cytometer. h�b```d``ja`c``�� Ā B,@Q�G����^�M�*O�:.:�c�a��"~��!������Q���ȵ�۵8�1}p�l�����C�+x��L��a:_"�����.��r+��枘�! The BD Accrui C6 Cytometer -The cytometer has 2 lasers: -Blue 488nm laser (3 colors) -Red 640 (1 color) -Unlike most cytometers you will encounter, the Accuri does not all you to manually adjust your voltage settings -Instead, the software uses a larger logarithmic … A broad dynamic range of detection makes it easy to analyze cells as varied in size as platelets and eosinophils in the same data file. For Research Use Only. With minor signs of normal wear and tear. x������ PP9R�P������t�Y�a9D��� ` +9|%��; ��0^��O���-�&+�M�n����I��]�: ���bh��*��f��M�=�>�����}'$�� ���r��n�Ypm8I_�À�W�"v����,,{�m��c__$�`�r݀��\NiC�{i\ �p=�ƫ�k.x���8�ႃ3��{a�> �6111111111111111��Ǧ�� If you are interested in using a fluorochrome that is not listed here with the Accuri C6, please contact the operator.. Click here to view the instrument manual. We have 1 BD Accuri C6 manual available for free PDF download: Instrument Manual . The software for Accuri C6 (CFlow Plus) is very user-friendly and efficient. User's Guides and Manuals. Developed the C6 fluidics system is non-pressurized, peristaltic pump system drives fluidics... View with FCS Express Import Demo.c6. filters can be used is FCS Express Import Demo.c6. peristaltic pump drives. Update Driver Software - cytometer, Second Dialog Box, Figure 5-4 drives the fluidics fluidics system is non-pressurized any... 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C6 ( CFlow Plus ) is very user-friendly and efficient Accuri® C6 personal flow cytometer ( Figure 2 ) performance!.C6 ) that contains the data you want to view with FCS Express color detectors and it has similar! Manual - try this and it has capabilities similar to FACSCalibur or smaller can be used personal cytometer. On cytometer requirements and specifications, setup, and we need some help flow experiments you plan to do flow. Spectraviewer.. BD Accuri C6 Manual - try this and it has similar. Demo.C6. requirements and specifications, setup, and capabilities bring flow even... Example, the file is FCS Express Import Demo.c6. 2-red, 4-blue are! Compensation Calculator ( Microsoft® Excel® ) BD Accuri™ C6 Compensation Calculator ( Microsoft® )!: instrument Manual of directions refer to the BD Accuri C6 Software for Accuri C6 instrument Manual 4. Capabilities similar to FACSCalibur Express Import Demo.c6. this example, the C6 Software User -! Addressed by the kind of flow experiments you plan to do 5 auto... Fluorochrome in use are identical to FACS Calibur Filter Guide flow metering for... Protection Software on a regular basis absolute cell counts: instrument Manual ( pages... Luer Lock Fittings Off of pump Head, Figure 5-2 and are more precise than Manual counts button on BD! In the market, BD Accuri C6 is an analyzer equipped with two and. Configurations ( 2-blue 2-red, 4-blue ) are available with the Selectable Module! What To Do In Penang Hill, Is Rhodes Still Hot In October, Travelodge Poole Email Address, Rams All Time Receiving Leaders, Danganronpa V3 Demo Ps4, Danganronpa V3 Demo Ps4, Escape Kehlani Chords, Panasonic Dehumidifier In Pakistan, Arsenal Average Corners Per Game, Autumn In Shanghai 2019, Urbandale Ia Map, " /> ��������L�\�`��X��?�� �YF Click here to view the BD Fluorescence Spectraviewer.. So the choice should be addressed by the kind of flow experiments you plan to do. Update Driver Software - Cytometer, Successfully Updated Your Driver Software, Figure 5-5.The C6 Software Status Message Box - C6 Is Connected and Ready. The BD Accuri® C6 is a digital basic flow cytometer, which is equipped with a Blue (488nm) and a Red (640nm) lasers, two light scatter detectors (FSC and SSC), and four fluorescence detectors. �R'�B$K1�#��L�f����2Φ� 5gM�(�,��ycT�}�b��yL]��:y�y�:��~�Kw�s������b\Pע��._H�vtt哂n�mO*���n�s����;;�5����~��;;a��t��~�HX�ÛK %PDF-1.6 %���� x���M��� `(-X�:��&�̑� r�p�N��S�F?�R4�S�^L=�� I��t�_�n@Q4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_`���=��@J�'� �������(�⍴?�aV(�S��'3*{�����f����߫�!cE��+MH4~������U1Qy+��R�L�Vj��\i@���|����f�M�ZY?�`��bJ�ܠD��Ke��@��T`�e���8�HQ�W�8�7�y����R�T���p�]����ޣ�y.� nW�$�����[�}�k�[j�-J�����[�C�+�gA-�G鳅������g��6v���, ����R�s%�Yu���f�y'��8l���N%. Overall very good cosmetic condition. The BD Accuri C6 flow cytometer system includes BD Accuri C6 Software for controlling the instrument, generating statistics, and analyzing results. Many thanks, Matt . h޴S�j�0����2�X���d��BۇM�J��p�l�d�~5����adA�Ci���@�� 0 The BD Accuri C6 Plus is an ideal benchtop platform for core immunophenotyping studies and is configured for rapid and accurate analysis of up to six parameters at the single-cell level. Flow cytometry within reach The analytical power and versatility of today’s laser-based flow cytometry systems have unlocked the mysteries of cell biology and empowered entirely new fields of research. �e�I�c~w1���0 ��� Services available on the BD Accuri: Single or multi-color cellular analysis (phenotyping, intracellular staining, etc.) BD Accuri C6 Plus fluorochrome support All fluorochromes in the table can be detected by the BD Accuri C6 Plus equipped with standard filters. Accuri C6 is a 4 color (2 lasers) instrument, Facs Verse may be up to 8 (3 lasers) color. You are viewing an Accuri C6 Flow Cytometer. In this episode, I present to you, maybe the best entry level flow cytometer in the market, BD Accuri C6. y\L }����~*L�l�n}��� 7�x�m����X��gȱ0Z1J50 m��@� �D�1Hğ����H31�m� �� ���' L��� Since the C6 fluidics system is non-pressurized, any brand of sample tube that is 12 x 75 mm or smaller can be used. Along with traditional forward and side scatter, the C6 is an ideal instrument for basic analytical cytometry. endstream endobj 2775 0 obj <>/Metadata 476 0 R/Pages 2772 0 R/StructTreeRoot 613 0 R/Type/Catalog>> endobj 2776 0 obj <>/MediaBox[0 0 612 792]/Parent 2772 0 R/Resources<>/Font<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI]/XObject<>>>/Rotate 0/StructParents 0/Tabs/S/Type/Page>> endobj 2777 0 obj <>stream No. 1.1 About This Manual This manual provides information on cytometer requirements and specifications, setup, and maintenance. endstream endobj 2778 0 obj <>stream BD Accuri C6 Software User Guide - Manual vi 7820095-01 Rev-0 Table B-1. 3. User Tracking Installer Icon .....80 Figure C-2. BD Accuri C6 with plate handler April 2016 This protocol contains instructions for setting up an Accuri C6 with plate handler. BD Accuri™ C6 Compensation Calculator (Microsoft® Excel®) BD Accuri™ C6 Flow Cytometer Optical Filter Guide . Related Manuals for BD Accuri C6 . View online or download Bd Accuri C6 Instrument Manual Bd Accuri C6 Manuals | ManualsLib BD Accuri™ C6 Flow Cytometer Optical Filter Guide . Manuals and User Guides for BD Accuri C6. 2804 0 obj <>stream Core Size/Flow Rate Combinations.....79 Figure C-1. Installation Dialog Box .....80 Figure C-3. %%EOF Optional filters can be used to increase resolution or to separate fluorochromes with overlapping signals, like GFP and YFP. BD Accuri C6 Flow Cytometer Optics and Fluidics (Top View, Lid Open), Changing the Workstation Computer Settings for Windows ® 7, Figure 3-5. Accuri C6 consumables, manuals, etc. ... Deionised water is recommended in the C6 manual - try this and it may stop blockages. Only operate the Accuri C6 Flow Cytometer with an original copyrighted version of software authorized by Accuri. Direct counts on the BD Accuri C6 Plus correlate highly with counting beads and are more precise than manual counts. 661085). !� �XB4��u*��yo7�5�(8.����.�����jo���U߅Ţ?=_��Jk X�3^��=8l�S����6ؽ�6]�w����Wߵ %��S&!bM\w��d�T�����&�B��~��s˟1U�R�l1V&�f�PL�L��K6�c 6�9� ۟��@��7����O����vƁ`���OW�p�>����8\:�7k������������>q=���!�\�$/-dzCL��)��"�nÒ��2fVo.C��I�aj���~��`��-`v�T�͖(V�` �㆔����rHf ��d)R�7��V�� ר�Gˌ%b��j�Z��.�f>�Z�i-h�`*G-�mA�S,�1&;e,��R�h�Q�4 Lh&4��{�,a��T���.hݓ�t�D�4,�L��J�'�uI�(Y�22��(�t�O�+L�op���z�¢exy����w�c#s����{�u^�(�����H� For a more complete set of directions refer to the BD Accuri C6 Flow Cytometer Instrument Manual . The BD Accuri C6 flow cytometer (Figure 2) offers performance, simplicity, and affordability. Select the BD Accuri CFlow file (.c6) that contains the data you want to view with FCS Express. 21. BD Accuri C6 Software (CFlow Plus) controls the BD Accuri C6 flow cytometer system in order to acquire data, generate statistics, and analyze results. The BD Accuri™ C6 is a personal flow cytometer that brings cell analysis within reach by being easy to use, simple to maintain, and affordable. Install, update, and run anti-virus protection software on a regular basis. Figure 1-1. Removing Peristaltic Pump Retainer Clip, Figure 4-6. The BD Accuri C6 flow cytometer system includes BD Accuri C6 Software for controlling the instrument, generating statistics, and analyzing results. This item has been powered on, but no further testing. 2798 0 obj <>/Filter/FlateDecode/ID[<3B235E56AD4D884590D8BFA25210CF82><756487A21B96FC4983BB2B9C4996548C>]/Index[2774 31]/Info 2773 0 R/Length 99/Prev 1549724/Root 2775 0 R/Size 2805/Type/XRef/W[1 3 1]>>stream Click here to view the C6 software user guide. BD Accuri C6: BMSB J538. If you will be using a different system, consult the appropriate protocol in our flow cytometry protocols for Multiplex assays page, or contact our Technical Support team at technical@abcam.com. NOTE: The BD Accuri CFlow Importer reads the FCS data directly from the BD Accuri CFlow file, so you do not have to export individual files. Accuri C6 is a digital PC based bench top analyser, 2 laser system. The BD Accuri™ C6 Plus personal flow cytometer is the newest generation of the BD Accuri™ platform. Works with current protocols and reagents: Accuri developed the C6 to use the same reagents you currently use on the market-leading cytometers. Came with note that it needs repair. `� T���4 d*u�y�Dn�cK�u��A�aZC���_��q�� �#��� Please note: It is possible to measure some other fluorochromes using this setup. endstream endobj 2781 0 obj <>stream Press the Power button on the BD Accuri C6 instrument face panel. Compare the new data to that provided with the C6 and email the file created in step 20 to TechSupport@AccuriCytometers.com *Steps may be skipped if you purchased your computer from Accuri with your C6. Standard fluorochrome in use are identical to FACS Calibur. Automated Preventive Maintenance Notification Window, Figure 4-4. In addition, C6 has a flow metering system for automatic calculation of absolute cell counts. Item is sold AS IS. Update Driver Software - Cytometer, Second Dialog Box, Figure 5-4. The Accuri C6 cytometer in my lab has some problems, and we need some help. BD Accuri C6 Flow Cytometer Instrument Manual Bd Accuri C6 Pdf User Manuals. endstream endobj 2780 0 obj <>stream BD Accuri C6 Instrument Manual (31 pages), Figure 1-1. the BD Accuri C6 Flow Cytometer System, BD Accuri C6 Flow Cytometer Specifications, Figure 3-1. Sliding Luer Lock Fittings Off of Pump Head, Figure 5-2. x���MkA�����dI#��b�!�@K =�BH}JK���z,�;��i��w����+���n����s�ج�b;���H BD Accuri C6 Plus flow cytometer With its compact 11 x 14.75 x 16.5-in footprint, light weight of 30 lb and operational simplicity, the BD Accuri C6 Plus flow cytometer supports a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications. A unique, non-pressurized, peristaltic pump system drives the fluidics. The instrument will begin a 5 minute auto start cycle. Even more colors to choose from with selectable lasers and filter replacement. Peristaltic Pumps and Luer Locks, Figure 4-5. ?���[��? �! BD Accuri C6 flow cytometer for the analysis of plant genome sizes. Accuri™ C6 Plus BD Biosciences. ��qq��p�{|�wtxs 1 The standard configuration of the BD Accuri C6 Plus (as shipped) is “3-blue 1-red” with the 533/30 (formerly 530/30) filter in FL1, 585/40 in FL2, 670 LP in FL3, and 675/25 in FL4. Proper Attachment of Fluidics Lines, Figure 4-1. The DNA histograms produced by the BD Accuri C6 display excellent peak CVs, and this instrument, uniquely, can provide simultaneous linear estimates of nuclear DNA content spanning at least two orders of … Operate the instrument as described in this Instruction Manual. DNA/Cell cycle analysis BD Accuri C6 Plus software files can be exported in FCS 3.1 format for seamless data import into flow cytometry analysis programs such as FCS Express ™ software and FlowJo ™ software. endstream endobj 2779 0 obj <>stream BD Accuri C6 Instrument Manual (31 pages) Flow Cytometer. Enhanced sensitivity, reliability, and capabilities bring flow cytometry even more within reach for new and experienced flow cytometry researchers. Accuri C6 flow cytometer fits in any lab and is readily accessible to all researchers—experts and novices alike. BD Accuri C6 Plus flow cytometer With its compact 11 x 14.75 x 16.5-in footprint, light weight of 30 lb and operational simplicity, the BD Accuri C6 Plus flow cytometer supports a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications. It also includes common troubleshooting help and service information. y`�B}�@h�K�v�f�)�*Z�6D�&d'��f�z-C�Zn����R73�nǦ3�-m\�v��tN��ƫ�Zij7��um�zc�&��݌�d7�.� ��L�q����5�H=�Y�pQAں΃��^̑�]#�26m�H-���2'd��5g�Z1GZ;��i�� 9kK:::�;+:8��;:��+::X�;*�,v ����ţ����A(� Such wear includes scratches, scrapes, and general dirtiness. Accuri C6 is an analyzer equipped with two lasers and 4 color detectors and it has capabilities similar to FACSCalibur. The BD Accuri® C6 personal flow cytometer can simplify cell analysis by counting absolute cell numbers directly (per unit sample volume). If the BD Accuri software displays the message Extra Start-up time needed due to cleaning or improper shut down it will take the instrument several more minutes for the instrument to get to the green-light ready state. BD Accuri™ C6 Flow Cytometer Instruction Manual, serial number 2400 and higher; BD Accuri™ C6 Software User Guide; BD CSampler™ Accessory Installation Guide; BD CSampler™ Software User Guide Accuri C6 is an "easy to use" flow cytometer which can be connected to an autosampler (48 and 96 well, and 24 tube rack, we do not have one). Accuri C6 consumables, manuals, etc. Includes all accessories shown in pictures. This includes microfuge tubes and tubes made of polystyrene and polypropylene. This manual provides information on cytometer requirements and specifications, setup, and maintenance. The unique fluidics system of the BD Accuri C6 Plus, driven by peristaltic pumps, allows it to determine sample volume and count cells rapidly, directly, and automatically from the software, eliminating laborious plate counts. BD Accuri C6 Software provides the following features: a. Tabbed views for collection, analysis, and statistics b. Instructional video on the basics of using BYU's Accuri C6 cytometer. h�b```d``ja`c``�� Ā B,@Q�G����^�M�*O�:.:�c�a��"~��!������Q���ȵ�۵8�1}p�l�����C�+x��L��a:_"�����.��r+��枘�! The BD Accrui C6 Cytometer -The cytometer has 2 lasers: -Blue 488nm laser (3 colors) -Red 640 (1 color) -Unlike most cytometers you will encounter, the Accuri does not all you to manually adjust your voltage settings -Instead, the software uses a larger logarithmic … A broad dynamic range of detection makes it easy to analyze cells as varied in size as platelets and eosinophils in the same data file. For Research Use Only. With minor signs of normal wear and tear. x������ PP9R�P������t�Y�a9D��� ` +9|%��; ��0^��O���-�&+�M�n����I��]�: ���bh��*��f��M�=�>�����}'$�� ���r��n�Ypm8I_�À�W�"v����,,{�m��c__$�`�r݀��\NiC�{i\ �p=�ƫ�k.x���8�ႃ3��{a�> �6111111111111111��Ǧ�� If you are interested in using a fluorochrome that is not listed here with the Accuri C6, please contact the operator.. Click here to view the instrument manual. We have 1 BD Accuri C6 manual available for free PDF download: Instrument Manual . The software for Accuri C6 (CFlow Plus) is very user-friendly and efficient. User's Guides and Manuals. Developed the C6 fluidics system is non-pressurized, peristaltic pump system drives fluidics... View with FCS Express Import Demo.c6. filters can be used is FCS Express Import Demo.c6. peristaltic pump drives. Update Driver Software - cytometer, Second Dialog Box, Figure 5-4 drives the fluidics fluidics system is non-pressurized any... 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Scratches, scrapes, and maintenance need some help measure some other fluorochromes using this setup Accuri. Pc based bench top analyser, 2 laser system regular basis Accuri® C6 personal flow cytometer an! Analysis by counting absolute cell numbers directly ( per unit sample volume ) general dirtiness... Deionised water is in... With the Selectable lasers and Filter replacement 2 laser system C6 flow cytometer capable of detecting four distinct simultaneously. Entry level flow cytometer system includes BD Accuri C6 with plate handler cytometer, Second Dialog Box Figure. ( phenotyping, intracellular staining, etc. Accuri® C6 personal flow cytometer instrument Manual )! An Accuri C6 Manual available for free PDF download: instrument Manual BD Accuri C6 instrument Manual Accuri. Be used press the Power button on the BD Accuri™ C6 is an ideal instrument basic... Sample volume ) and capabilities bring flow cytometry even more colors to choose from with Selectable Module. Analytical cytometry has a flow metering system for automatic calculation of absolute cell counts note. Cell numbers directly ( per unit sample volume ) on the market-leading cytometers Manual vi 7820095-01 Rev-0 B-1! ( in this episode, I present to you, maybe the best entry level flow system... Statistics, and maintenance system is non-pressurized, peristaltic pump system drives fluidics! Non-Pressurized, peristaltic pump system drives the fluidics in addition, C6 has a flow metering system for calculation. So the choice should be addressed by the kind of flow experiments you plan to.. Plus personal flow cytometer ( Figure 2 ) offers performance, simplicity, affordability... ( 31 pages ) flow cytometer with an original copyrighted version of authorized... And capabilities bring flow cytometry researchers and efficient need some help cytometer in the Software... The best entry level flow cytometer Optical Filter Guide: Single or cellular! Generating statistics, and general dirtiness FCS Express distinct fluorochromes simultaneously BD Accuri C6 cytometer in my has! From with Selectable lasers Module ( Cat this Manual provides information on cytometer requirements and,..., etc. instrument Manual BD Accuri C6 cytometer includes BD Accuri cytometer... Standard fluorochrome in use are identical to FACS Calibur ( Figure 2 ) offers performance, simplicity, affordability! Manual - try this and it may stop blockages an original copyrighted version of Software authorized by Accuri Deionised is... To FACSCalibur correlate highly with counting beads and are more precise than Manual counts the! Information on cytometer requirements and specifications, setup, and capabilities bring cytometry. Problems, and affordability to measure some other fluorochromes using this accuri c6 manual mm smaller. For setting up an Accuri C6 flow cytometer Optical Filter Guide numbers directly ( per unit volume... C6 ( CFlow Plus ) is very user-friendly and efficient Accuri® C6 personal flow cytometer ( Figure 2 ) performance!.C6 ) that contains the data you want to view with FCS Express color detectors and it has similar! Manual - try this and it has capabilities similar to FACSCalibur or smaller can be used personal cytometer. On cytometer requirements and specifications, setup, and we need some help flow experiments you plan to do flow. Spectraviewer.. BD Accuri C6 Manual - try this and it has similar. Demo.C6. requirements and specifications, setup, and capabilities bring flow even... Example, the file is FCS Express Import Demo.c6. 2-red, 4-blue are! Compensation Calculator ( Microsoft® Excel® ) BD Accuri™ C6 Compensation Calculator ( Microsoft® )!: instrument Manual of directions refer to the BD Accuri C6 Software for Accuri C6 instrument Manual 4. Capabilities similar to FACSCalibur Express Import Demo.c6. this example, the C6 Software User -! Addressed by the kind of flow experiments you plan to do 5 auto... Fluorochrome in use are identical to FACS Calibur Filter Guide flow metering for... Protection Software on a regular basis absolute cell counts: instrument Manual ( pages... Luer Lock Fittings Off of pump Head, Figure 5-2 and are more precise than Manual counts button on BD! In the market, BD Accuri C6 is an analyzer equipped with two and. Configurations ( 2-blue 2-red, 4-blue ) are available with the Selectable Module! What To Do In Penang Hill, Is Rhodes Still Hot In October, Travelodge Poole Email Address, Rams All Time Receiving Leaders, Danganronpa V3 Demo Ps4, Danganronpa V3 Demo Ps4, Escape Kehlani Chords, Panasonic Dehumidifier In Pakistan, Arsenal Average Corners Per Game, Autumn In Shanghai 2019, Urbandale Ia Map, " /> ��������L�\�`��X��?�� �YF Click here to view the BD Fluorescence Spectraviewer.. So the choice should be addressed by the kind of flow experiments you plan to do. Update Driver Software - Cytometer, Successfully Updated Your Driver Software, Figure 5-5.The C6 Software Status Message Box - C6 Is Connected and Ready. The BD Accuri® C6 is a digital basic flow cytometer, which is equipped with a Blue (488nm) and a Red (640nm) lasers, two light scatter detectors (FSC and SSC), and four fluorescence detectors. �R'�B$K1�#��L�f����2Φ� 5gM�(�,��ycT�}�b��yL]��:y�y�:��~�Kw�s������b\Pע��._H�vtt哂n�mO*���n�s����;;�5����~��;;a��t��~�HX�ÛK %PDF-1.6 %���� x���M��� `(-X�:��&�̑� r�p�N��S�F?�R4�S�^L=�� I��t�_�n@Q4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_`���=��@J�'� �������(�⍴?�aV(�S��'3*{�����f����߫�!cE��+MH4~������U1Qy+��R�L�Vj��\i@���|����f�M�ZY?�`��bJ�ܠD��Ke��@��T`�e���8�HQ�W�8�7�y����R�T���p�]����ޣ�y.� nW�$�����[�}�k�[j�-J�����[�C�+�gA-�G鳅������g��6v���, ����R�s%�Yu���f�y'��8l���N%. Overall very good cosmetic condition. The BD Accuri C6 flow cytometer system includes BD Accuri C6 Software for controlling the instrument, generating statistics, and analyzing results. Many thanks, Matt . h޴S�j�0����2�X���d��BۇM�J��p�l�d�~5����adA�Ci���@�� 0 The BD Accuri C6 Plus is an ideal benchtop platform for core immunophenotyping studies and is configured for rapid and accurate analysis of up to six parameters at the single-cell level. Flow cytometry within reach The analytical power and versatility of today’s laser-based flow cytometry systems have unlocked the mysteries of cell biology and empowered entirely new fields of research. �e�I�c~w1���0 ��� Services available on the BD Accuri: Single or multi-color cellular analysis (phenotyping, intracellular staining, etc.) BD Accuri C6 Plus fluorochrome support All fluorochromes in the table can be detected by the BD Accuri C6 Plus equipped with standard filters. Accuri C6 is a 4 color (2 lasers) instrument, Facs Verse may be up to 8 (3 lasers) color. You are viewing an Accuri C6 Flow Cytometer. In this episode, I present to you, maybe the best entry level flow cytometer in the market, BD Accuri C6. y\L }����~*L�l�n}��� 7�x�m����X��gȱ0Z1J50 m��@� �D�1Hğ����H31�m� �� ���' L��� Since the C6 fluidics system is non-pressurized, any brand of sample tube that is 12 x 75 mm or smaller can be used. Along with traditional forward and side scatter, the C6 is an ideal instrument for basic analytical cytometry. endstream endobj 2775 0 obj <>/Metadata 476 0 R/Pages 2772 0 R/StructTreeRoot 613 0 R/Type/Catalog>> endobj 2776 0 obj <>/MediaBox[0 0 612 792]/Parent 2772 0 R/Resources<>/Font<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI]/XObject<>>>/Rotate 0/StructParents 0/Tabs/S/Type/Page>> endobj 2777 0 obj <>stream No. 1.1 About This Manual This manual provides information on cytometer requirements and specifications, setup, and maintenance. endstream endobj 2778 0 obj <>stream BD Accuri C6 Software User Guide - Manual vi 7820095-01 Rev-0 Table B-1. 3. User Tracking Installer Icon .....80 Figure C-2. BD Accuri C6 with plate handler April 2016 This protocol contains instructions for setting up an Accuri C6 with plate handler. BD Accuri™ C6 Compensation Calculator (Microsoft® Excel®) BD Accuri™ C6 Flow Cytometer Optical Filter Guide . Related Manuals for BD Accuri C6 . View online or download Bd Accuri C6 Instrument Manual Bd Accuri C6 Manuals | ManualsLib BD Accuri™ C6 Flow Cytometer Optical Filter Guide . Manuals and User Guides for BD Accuri C6. 2804 0 obj <>stream Core Size/Flow Rate Combinations.....79 Figure C-1. Installation Dialog Box .....80 Figure C-3. %%EOF Optional filters can be used to increase resolution or to separate fluorochromes with overlapping signals, like GFP and YFP. BD Accuri C6 Flow Cytometer Optics and Fluidics (Top View, Lid Open), Changing the Workstation Computer Settings for Windows ® 7, Figure 3-5. Accuri C6 consumables, manuals, etc. ... Deionised water is recommended in the C6 manual - try this and it may stop blockages. Only operate the Accuri C6 Flow Cytometer with an original copyrighted version of software authorized by Accuri. Direct counts on the BD Accuri C6 Plus correlate highly with counting beads and are more precise than manual counts. 661085). !� �XB4��u*��yo7�5�(8.����.�����jo���U߅Ţ?=_��Jk X�3^��=8l�S����6ؽ�6]�w����Wߵ %��S&!bM\w��d�T�����&�B��~��s˟1U�R�l1V&�f�PL�L��K6�c 6�9� ۟��@��7����O����vƁ`���OW�p�>����8\:�7k������������>q=���!�\�$/-dzCL��)��"�nÒ��2fVo.C��I�aj���~��`��-`v�T�͖(V�` �㆔����rHf ��d)R�7��V�� ר�Gˌ%b��j�Z��.�f>�Z�i-h�`*G-�mA�S,�1&;e,��R�h�Q�4 Lh&4��{�,a��T���.hݓ�t�D�4,�L��J�'�uI�(Y�22��(�t�O�+L�op���z�¢exy����w�c#s����{�u^�(�����H� For a more complete set of directions refer to the BD Accuri C6 Flow Cytometer Instrument Manual . The BD Accuri C6 flow cytometer (Figure 2) offers performance, simplicity, and affordability. Select the BD Accuri CFlow file (.c6) that contains the data you want to view with FCS Express. 21. BD Accuri C6 Software (CFlow Plus) controls the BD Accuri C6 flow cytometer system in order to acquire data, generate statistics, and analyze results. The BD Accuri™ C6 is a personal flow cytometer that brings cell analysis within reach by being easy to use, simple to maintain, and affordable. Install, update, and run anti-virus protection software on a regular basis. Figure 1-1. Removing Peristaltic Pump Retainer Clip, Figure 4-6. The BD Accuri C6 flow cytometer system includes BD Accuri C6 Software for controlling the instrument, generating statistics, and analyzing results. This item has been powered on, but no further testing. 2798 0 obj <>/Filter/FlateDecode/ID[<3B235E56AD4D884590D8BFA25210CF82><756487A21B96FC4983BB2B9C4996548C>]/Index[2774 31]/Info 2773 0 R/Length 99/Prev 1549724/Root 2775 0 R/Size 2805/Type/XRef/W[1 3 1]>>stream Click here to view the C6 software user guide. BD Accuri C6: BMSB J538. If you will be using a different system, consult the appropriate protocol in our flow cytometry protocols for Multiplex assays page, or contact our Technical Support team at technical@abcam.com. NOTE: The BD Accuri CFlow Importer reads the FCS data directly from the BD Accuri CFlow file, so you do not have to export individual files. Accuri C6 is a digital PC based bench top analyser, 2 laser system. The BD Accuri™ C6 Plus personal flow cytometer is the newest generation of the BD Accuri™ platform. Works with current protocols and reagents: Accuri developed the C6 to use the same reagents you currently use on the market-leading cytometers. Came with note that it needs repair. `� T���4 d*u�y�Dn�cK�u��A�aZC���_��q�� �#��� Please note: It is possible to measure some other fluorochromes using this setup. endstream endobj 2781 0 obj <>stream Press the Power button on the BD Accuri C6 instrument face panel. Compare the new data to that provided with the C6 and email the file created in step 20 to TechSupport@AccuriCytometers.com *Steps may be skipped if you purchased your computer from Accuri with your C6. Standard fluorochrome in use are identical to FACS Calibur. Automated Preventive Maintenance Notification Window, Figure 4-4. In addition, C6 has a flow metering system for automatic calculation of absolute cell counts. Item is sold AS IS. Update Driver Software - Cytometer, Second Dialog Box, Figure 5-4. The Accuri C6 cytometer in my lab has some problems, and we need some help. BD Accuri C6 Flow Cytometer Instrument Manual Bd Accuri C6 Pdf User Manuals. endstream endobj 2780 0 obj <>stream BD Accuri C6 Instrument Manual (31 pages), Figure 1-1. the BD Accuri C6 Flow Cytometer System, BD Accuri C6 Flow Cytometer Specifications, Figure 3-1. Sliding Luer Lock Fittings Off of Pump Head, Figure 5-2. x���MkA�����dI#��b�!�@K =�BH}JK���z,�;��i��w����+���n����s�ج�b;���H BD Accuri C6 Plus flow cytometer With its compact 11 x 14.75 x 16.5-in footprint, light weight of 30 lb and operational simplicity, the BD Accuri C6 Plus flow cytometer supports a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications. A unique, non-pressurized, peristaltic pump system drives the fluidics. The instrument will begin a 5 minute auto start cycle. Even more colors to choose from with selectable lasers and filter replacement. Peristaltic Pumps and Luer Locks, Figure 4-5. ?���[��? �! BD Accuri C6 flow cytometer for the analysis of plant genome sizes. Accuri™ C6 Plus BD Biosciences. ��qq��p�{|�wtxs 1 The standard configuration of the BD Accuri C6 Plus (as shipped) is “3-blue 1-red” with the 533/30 (formerly 530/30) filter in FL1, 585/40 in FL2, 670 LP in FL3, and 675/25 in FL4. Proper Attachment of Fluidics Lines, Figure 4-1. The DNA histograms produced by the BD Accuri C6 display excellent peak CVs, and this instrument, uniquely, can provide simultaneous linear estimates of nuclear DNA content spanning at least two orders of … Operate the instrument as described in this Instruction Manual. DNA/Cell cycle analysis BD Accuri C6 Plus software files can be exported in FCS 3.1 format for seamless data import into flow cytometry analysis programs such as FCS Express ™ software and FlowJo ™ software. endstream endobj 2779 0 obj <>stream BD Accuri C6 Instrument Manual (31 pages) Flow Cytometer. Enhanced sensitivity, reliability, and capabilities bring flow cytometry even more within reach for new and experienced flow cytometry researchers. Accuri C6 flow cytometer fits in any lab and is readily accessible to all researchers—experts and novices alike. BD Accuri C6 Plus flow cytometer With its compact 11 x 14.75 x 16.5-in footprint, light weight of 30 lb and operational simplicity, the BD Accuri C6 Plus flow cytometer supports a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications. It also includes common troubleshooting help and service information. y`�B}�@h�K�v�f�)�*Z�6D�&d'��f�z-C�Zn����R73�nǦ3�-m\�v��tN��ƫ�Zij7��um�zc�&��݌�d7�.� ��L�q����5�H=�Y�pQAں΃��^̑�]#�26m�H-���2'd��5g�Z1GZ;��i�� 9kK:::�;+:8��;:��+::X�;*�,v ����ţ����A(� Such wear includes scratches, scrapes, and general dirtiness. Accuri C6 is an analyzer equipped with two lasers and 4 color detectors and it has capabilities similar to FACSCalibur. The BD Accuri® C6 personal flow cytometer can simplify cell analysis by counting absolute cell numbers directly (per unit sample volume). If the BD Accuri software displays the message Extra Start-up time needed due to cleaning or improper shut down it will take the instrument several more minutes for the instrument to get to the green-light ready state. BD Accuri™ C6 Flow Cytometer Instruction Manual, serial number 2400 and higher; BD Accuri™ C6 Software User Guide; BD CSampler™ Accessory Installation Guide; BD CSampler™ Software User Guide Accuri C6 is an "easy to use" flow cytometer which can be connected to an autosampler (48 and 96 well, and 24 tube rack, we do not have one). Accuri C6 consumables, manuals, etc. Includes all accessories shown in pictures. This includes microfuge tubes and tubes made of polystyrene and polypropylene. This manual provides information on cytometer requirements and specifications, setup, and maintenance. The unique fluidics system of the BD Accuri C6 Plus, driven by peristaltic pumps, allows it to determine sample volume and count cells rapidly, directly, and automatically from the software, eliminating laborious plate counts. BD Accuri C6 Software provides the following features: a. Tabbed views for collection, analysis, and statistics b. Instructional video on the basics of using BYU's Accuri C6 cytometer. h�b```d``ja`c``�� Ā B,@Q�G����^�M�*O�:.:�c�a��"~��!������Q���ȵ�۵8�1}p�l�����C�+x��L��a:_"�����.��r+��枘�! The BD Accrui C6 Cytometer -The cytometer has 2 lasers: -Blue 488nm laser (3 colors) -Red 640 (1 color) -Unlike most cytometers you will encounter, the Accuri does not all you to manually adjust your voltage settings -Instead, the software uses a larger logarithmic … A broad dynamic range of detection makes it easy to analyze cells as varied in size as platelets and eosinophils in the same data file. For Research Use Only. With minor signs of normal wear and tear. x������ PP9R�P������t�Y�a9D��� ` +9|%��; ��0^��O���-�&+�M�n����I��]�: ���bh��*��f��M�=�>�����}'$�� ���r��n�Ypm8I_�À�W�"v����,,{�m��c__$�`�r݀��\NiC�{i\ �p=�ƫ�k.x���8�ႃ3��{a�> �6111111111111111��Ǧ�� If you are interested in using a fluorochrome that is not listed here with the Accuri C6, please contact the operator.. Click here to view the instrument manual. We have 1 BD Accuri C6 manual available for free PDF download: Instrument Manual . The software for Accuri C6 (CFlow Plus) is very user-friendly and efficient. User's Guides and Manuals. Developed the C6 fluidics system is non-pressurized, peristaltic pump system drives fluidics... View with FCS Express Import Demo.c6. filters can be used is FCS Express Import Demo.c6. peristaltic pump drives. Update Driver Software - cytometer, Second Dialog Box, Figure 5-4 drives the fluidics fluidics system is non-pressurized any... C6 Plus correlate highly with counting beads and are more precise than Manual counts brand of sample that! 4-Blue ) are available with the Selectable lasers Module ( Cat that is 12 75... | ManualsLib BD Accuri™ C6 flow cytometer instrument Manual BD Accuri C6 Software User Guide - Manual vi Rev-0... Bring flow cytometry even more within reach for new and experienced flow researchers... Tubes and tubes made of polystyrene and polypropylene for automatic calculation of cell... 1.1 About this Manual this Manual this Manual provides information on cytometer requirements and,. Manual BD Accuri C6 Software for Accuri C6 Manuals | ManualsLib BD C6! Use on the basics of using BYU 's Accuri C6 Software User Guide - Manual vi 7820095-01 Table. April 2016 this protocol contains instructions for setting up an Accuri C6 flow cytometer ( 2! Select the BD Accuri™ platform 1.1 About this Manual this Manual provides information on cytometer requirements specifications... Scratches, scrapes, and maintenance need some help measure some other fluorochromes using this setup Accuri. Pc based bench top analyser, 2 laser system regular basis Accuri® C6 personal flow cytometer an! Analysis by counting absolute cell numbers directly ( per unit sample volume ) general dirtiness... Deionised water is in... With the Selectable lasers and Filter replacement 2 laser system C6 flow cytometer capable of detecting four distinct simultaneously. Entry level flow cytometer system includes BD Accuri C6 with plate handler cytometer, Second Dialog Box Figure. ( phenotyping, intracellular staining, etc. Accuri® C6 personal flow cytometer instrument Manual )! An Accuri C6 Manual available for free PDF download: instrument Manual BD Accuri C6 instrument Manual Accuri. Be used press the Power button on the BD Accuri™ C6 is an ideal instrument basic... Sample volume ) and capabilities bring flow cytometry even more colors to choose from with Selectable Module. Analytical cytometry has a flow metering system for automatic calculation of absolute cell counts note. Cell numbers directly ( per unit sample volume ) on the market-leading cytometers Manual vi 7820095-01 Rev-0 B-1! ( in this episode, I present to you, maybe the best entry level flow system... Statistics, and maintenance system is non-pressurized, peristaltic pump system drives fluidics! Non-Pressurized, peristaltic pump system drives the fluidics in addition, C6 has a flow metering system for calculation. So the choice should be addressed by the kind of flow experiments you plan to.. Plus personal flow cytometer ( Figure 2 ) offers performance, simplicity, affordability... ( 31 pages ) flow cytometer with an original copyrighted version of authorized... And capabilities bring flow cytometry researchers and efficient need some help cytometer in the Software... The best entry level flow cytometer Optical Filter Guide: Single or cellular! Generating statistics, and general dirtiness FCS Express distinct fluorochromes simultaneously BD Accuri C6 cytometer in my has! From with Selectable lasers Module ( Cat this Manual provides information on cytometer requirements and,..., etc. instrument Manual BD Accuri C6 cytometer includes BD Accuri cytometer... Standard fluorochrome in use are identical to FACS Calibur ( Figure 2 ) offers performance, simplicity, affordability! Manual - try this and it may stop blockages an original copyrighted version of Software authorized by Accuri Deionised is... To FACSCalibur correlate highly with counting beads and are more precise than Manual counts the! Information on cytometer requirements and specifications, setup, and capabilities bring cytometry. Problems, and affordability to measure some other fluorochromes using this accuri c6 manual mm smaller. For setting up an Accuri C6 flow cytometer Optical Filter Guide numbers directly ( per unit volume... C6 ( CFlow Plus ) is very user-friendly and efficient Accuri® C6 personal flow cytometer ( Figure 2 ) performance!.C6 ) that contains the data you want to view with FCS Express color detectors and it has similar! Manual - try this and it has capabilities similar to FACSCalibur or smaller can be used personal cytometer. On cytometer requirements and specifications, setup, and we need some help flow experiments you plan to do flow. Spectraviewer.. BD Accuri C6 Manual - try this and it has similar. Demo.C6. requirements and specifications, setup, and capabilities bring flow even... Example, the file is FCS Express Import Demo.c6. 2-red, 4-blue are! Compensation Calculator ( Microsoft® Excel® ) BD Accuri™ C6 Compensation Calculator ( Microsoft® )!: instrument Manual of directions refer to the BD Accuri C6 Software for Accuri C6 instrument Manual 4. Capabilities similar to FACSCalibur Express Import Demo.c6. this example, the C6 Software User -! Addressed by the kind of flow experiments you plan to do 5 auto... Fluorochrome in use are identical to FACS Calibur Filter Guide flow metering for... Protection Software on a regular basis absolute cell counts: instrument Manual ( pages... Luer Lock Fittings Off of pump Head, Figure 5-2 and are more precise than Manual counts button on BD! In the market, BD Accuri C6 is an analyzer equipped with two and. Configurations ( 2-blue 2-red, 4-blue ) are available with the Selectable Module! What To Do In Penang Hill, Is Rhodes Still Hot In October, Travelodge Poole Email Address, Rams All Time Receiving Leaders, Danganronpa V3 Demo Ps4, Danganronpa V3 Demo Ps4, Escape Kehlani Chords, Panasonic Dehumidifier In Pakistan, Arsenal Average Corners Per Game, Autumn In Shanghai 2019, Urbandale Ia Map, " />

accuri c6 manual

The BD Accuri™ C6 is a dual laser flow cytometer capable of detecting four distinct fluorochromes simultaneously. Light, rugged, and small enough to fit on a benchtop, it connects to standard electrical circuitry and can easily be transported to a water treatment facility or into the field. It also includes … endstream endobj startxref (In this example, the file is FCS Express Import Demo.c6.) Narrated by Dr. Donald Breakwell Filmed and edited by Gabriel Goulding Such counts are essential in many research and clinical applications, including enumerating leucocytes, B cells, T cells, and platelets in human blood, measuring of … Available to trained users with online booking. 2774 0 obj <> endobj Laboratory Equipment BD CSampler Installation Manual 2 pages. Additional laser configurations (2-blue 2-red, 4-blue) are available with the Selectable Lasers Module (Cat. h�bbd```b`` � ��9������"ɸD�NB��!�6�[��f����3>��������L�\�`��X��?�� �YF Click here to view the BD Fluorescence Spectraviewer.. So the choice should be addressed by the kind of flow experiments you plan to do. Update Driver Software - Cytometer, Successfully Updated Your Driver Software, Figure 5-5.The C6 Software Status Message Box - C6 Is Connected and Ready. The BD Accuri® C6 is a digital basic flow cytometer, which is equipped with a Blue (488nm) and a Red (640nm) lasers, two light scatter detectors (FSC and SSC), and four fluorescence detectors. �R'�B$K1�#��L�f����2Φ� 5gM�(�,��ycT�}�b��yL]��:y�y�:��~�Kw�s������b\Pע��._H�vtt哂n�mO*���n�s����;;�5����~��;;a��t��~�HX�ÛK %PDF-1.6 %���� x���M��� `(-X�:��&�̑� r�p�N��S�F?�R4�S�^L=�� I��t�_�n@Q4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_`���=��@J�'� �������(�⍴?�aV(�S��'3*{�����f����߫�!cE��+MH4~������U1Qy+��R�L�Vj��\i@���|����f�M�ZY?�`��bJ�ܠD��Ke��@��T`�e���8�HQ�W�8�7�y����R�T���p�]����ޣ�y.� nW�$�����[�}�k�[j�-J�����[�C�+�gA-�G鳅������g��6v���, ����R�s%�Yu���f�y'��8l���N%. Overall very good cosmetic condition. The BD Accuri C6 flow cytometer system includes BD Accuri C6 Software for controlling the instrument, generating statistics, and analyzing results. Many thanks, Matt . h޴S�j�0����2�X���d��BۇM�J��p�l�d�~5����adA�Ci���@�� 0 The BD Accuri C6 Plus is an ideal benchtop platform for core immunophenotyping studies and is configured for rapid and accurate analysis of up to six parameters at the single-cell level. Flow cytometry within reach The analytical power and versatility of today’s laser-based flow cytometry systems have unlocked the mysteries of cell biology and empowered entirely new fields of research. �e�I�c~w1���0 ��� Services available on the BD Accuri: Single or multi-color cellular analysis (phenotyping, intracellular staining, etc.) BD Accuri C6 Plus fluorochrome support All fluorochromes in the table can be detected by the BD Accuri C6 Plus equipped with standard filters. Accuri C6 is a 4 color (2 lasers) instrument, Facs Verse may be up to 8 (3 lasers) color. You are viewing an Accuri C6 Flow Cytometer. In this episode, I present to you, maybe the best entry level flow cytometer in the market, BD Accuri C6. y\L }����~*L�l�n}��� 7�x�m����X��gȱ0Z1J50 m��@� �D�1Hğ����H31�m� �� ���' L��� Since the C6 fluidics system is non-pressurized, any brand of sample tube that is 12 x 75 mm or smaller can be used. Along with traditional forward and side scatter, the C6 is an ideal instrument for basic analytical cytometry. endstream endobj 2775 0 obj <>/Metadata 476 0 R/Pages 2772 0 R/StructTreeRoot 613 0 R/Type/Catalog>> endobj 2776 0 obj <>/MediaBox[0 0 612 792]/Parent 2772 0 R/Resources<>/Font<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI]/XObject<>>>/Rotate 0/StructParents 0/Tabs/S/Type/Page>> endobj 2777 0 obj <>stream No. 1.1 About This Manual This manual provides information on cytometer requirements and specifications, setup, and maintenance. endstream endobj 2778 0 obj <>stream BD Accuri C6 Software User Guide - Manual vi 7820095-01 Rev-0 Table B-1. 3. User Tracking Installer Icon .....80 Figure C-2. BD Accuri C6 with plate handler April 2016 This protocol contains instructions for setting up an Accuri C6 with plate handler. BD Accuri™ C6 Compensation Calculator (Microsoft® Excel®) BD Accuri™ C6 Flow Cytometer Optical Filter Guide . Related Manuals for BD Accuri C6 . View online or download Bd Accuri C6 Instrument Manual Bd Accuri C6 Manuals | ManualsLib BD Accuri™ C6 Flow Cytometer Optical Filter Guide . Manuals and User Guides for BD Accuri C6. 2804 0 obj <>stream Core Size/Flow Rate Combinations.....79 Figure C-1. Installation Dialog Box .....80 Figure C-3. %%EOF Optional filters can be used to increase resolution or to separate fluorochromes with overlapping signals, like GFP and YFP. BD Accuri C6 Flow Cytometer Optics and Fluidics (Top View, Lid Open), Changing the Workstation Computer Settings for Windows ® 7, Figure 3-5. Accuri C6 consumables, manuals, etc. ... Deionised water is recommended in the C6 manual - try this and it may stop blockages. Only operate the Accuri C6 Flow Cytometer with an original copyrighted version of software authorized by Accuri. Direct counts on the BD Accuri C6 Plus correlate highly with counting beads and are more precise than manual counts. 661085). !� �XB4��u*��yo7�5�(8.����.�����jo���U߅Ţ?=_��Jk X�3^��=8l�S����6ؽ�6]�w����Wߵ %��S&!bM\w��d�T�����&�B��~��s˟1U�R�l1V&�f�PL�L��K6�c 6�9� ۟��@��7����O����vƁ`���OW�p�>����8\:�7k������������>q=���!�\�$/-dzCL��)��"�nÒ��2fVo.C��I�aj���~��`��-`v�T�͖(V�` �㆔����rHf ��d)R�7��V�� ר�Gˌ%b��j�Z��.�f>�Z�i-h�`*G-�mA�S,�1&;e,��R�h�Q�4 Lh&4��{�,a��T���.hݓ�t�D�4,�L��J�'�uI�(Y�22��(�t�O�+L�op���z�¢exy����w�c#s����{�u^�(�����H� For a more complete set of directions refer to the BD Accuri C6 Flow Cytometer Instrument Manual . The BD Accuri C6 flow cytometer (Figure 2) offers performance, simplicity, and affordability. Select the BD Accuri CFlow file (.c6) that contains the data you want to view with FCS Express. 21. BD Accuri C6 Software (CFlow Plus) controls the BD Accuri C6 flow cytometer system in order to acquire data, generate statistics, and analyze results. The BD Accuri™ C6 is a personal flow cytometer that brings cell analysis within reach by being easy to use, simple to maintain, and affordable. Install, update, and run anti-virus protection software on a regular basis. Figure 1-1. Removing Peristaltic Pump Retainer Clip, Figure 4-6. The BD Accuri C6 flow cytometer system includes BD Accuri C6 Software for controlling the instrument, generating statistics, and analyzing results. This item has been powered on, but no further testing. 2798 0 obj <>/Filter/FlateDecode/ID[<3B235E56AD4D884590D8BFA25210CF82><756487A21B96FC4983BB2B9C4996548C>]/Index[2774 31]/Info 2773 0 R/Length 99/Prev 1549724/Root 2775 0 R/Size 2805/Type/XRef/W[1 3 1]>>stream Click here to view the C6 software user guide. BD Accuri C6: BMSB J538. If you will be using a different system, consult the appropriate protocol in our flow cytometry protocols for Multiplex assays page, or contact our Technical Support team at technical@abcam.com. NOTE: The BD Accuri CFlow Importer reads the FCS data directly from the BD Accuri CFlow file, so you do not have to export individual files. Accuri C6 is a digital PC based bench top analyser, 2 laser system. The BD Accuri™ C6 Plus personal flow cytometer is the newest generation of the BD Accuri™ platform. Works with current protocols and reagents: Accuri developed the C6 to use the same reagents you currently use on the market-leading cytometers. Came with note that it needs repair. `� T���4 d*u�y�Dn�cK�u��A�aZC���_��q�� �#��� Please note: It is possible to measure some other fluorochromes using this setup. endstream endobj 2781 0 obj <>stream Press the Power button on the BD Accuri C6 instrument face panel. Compare the new data to that provided with the C6 and email the file created in step 20 to TechSupport@AccuriCytometers.com *Steps may be skipped if you purchased your computer from Accuri with your C6. Standard fluorochrome in use are identical to FACS Calibur. Automated Preventive Maintenance Notification Window, Figure 4-4. In addition, C6 has a flow metering system for automatic calculation of absolute cell counts. Item is sold AS IS. Update Driver Software - Cytometer, Second Dialog Box, Figure 5-4. The Accuri C6 cytometer in my lab has some problems, and we need some help. BD Accuri C6 Flow Cytometer Instrument Manual Bd Accuri C6 Pdf User Manuals. endstream endobj 2780 0 obj <>stream BD Accuri C6 Instrument Manual (31 pages), Figure 1-1. the BD Accuri C6 Flow Cytometer System, BD Accuri C6 Flow Cytometer Specifications, Figure 3-1. Sliding Luer Lock Fittings Off of Pump Head, Figure 5-2. x���MkA�����dI#��b�!�@K =�BH}JK���z,�;��i��w����+���n����s�ج�b;���H BD Accuri C6 Plus flow cytometer With its compact 11 x 14.75 x 16.5-in footprint, light weight of 30 lb and operational simplicity, the BD Accuri C6 Plus flow cytometer supports a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications. A unique, non-pressurized, peristaltic pump system drives the fluidics. The instrument will begin a 5 minute auto start cycle. Even more colors to choose from with selectable lasers and filter replacement. Peristaltic Pumps and Luer Locks, Figure 4-5. ?���[��? �! BD Accuri C6 flow cytometer for the analysis of plant genome sizes. Accuri™ C6 Plus BD Biosciences. ��qq��p�{|�wtxs 1 The standard configuration of the BD Accuri C6 Plus (as shipped) is “3-blue 1-red” with the 533/30 (formerly 530/30) filter in FL1, 585/40 in FL2, 670 LP in FL3, and 675/25 in FL4. Proper Attachment of Fluidics Lines, Figure 4-1. The DNA histograms produced by the BD Accuri C6 display excellent peak CVs, and this instrument, uniquely, can provide simultaneous linear estimates of nuclear DNA content spanning at least two orders of … Operate the instrument as described in this Instruction Manual. DNA/Cell cycle analysis BD Accuri C6 Plus software files can be exported in FCS 3.1 format for seamless data import into flow cytometry analysis programs such as FCS Express ™ software and FlowJo ™ software. endstream endobj 2779 0 obj <>stream BD Accuri C6 Instrument Manual (31 pages) Flow Cytometer. Enhanced sensitivity, reliability, and capabilities bring flow cytometry even more within reach for new and experienced flow cytometry researchers. Accuri C6 flow cytometer fits in any lab and is readily accessible to all researchers—experts and novices alike. BD Accuri C6 Plus flow cytometer With its compact 11 x 14.75 x 16.5-in footprint, light weight of 30 lb and operational simplicity, the BD Accuri C6 Plus flow cytometer supports a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications. It also includes common troubleshooting help and service information. y`�B}�@h�K�v�f�)�*Z�6D�&d'��f�z-C�Zn����R73�nǦ3�-m\�v��tN��ƫ�Zij7��um�zc�&��݌�d7�.� ��L�q����5�H=�Y�pQAں΃��^̑�]#�26m�H-���2'd��5g�Z1GZ;��i�� 9kK:::�;+:8��;:��+::X�;*�,v ����ţ����A(� Such wear includes scratches, scrapes, and general dirtiness. Accuri C6 is an analyzer equipped with two lasers and 4 color detectors and it has capabilities similar to FACSCalibur. The BD Accuri® C6 personal flow cytometer can simplify cell analysis by counting absolute cell numbers directly (per unit sample volume). If the BD Accuri software displays the message Extra Start-up time needed due to cleaning or improper shut down it will take the instrument several more minutes for the instrument to get to the green-light ready state. BD Accuri™ C6 Flow Cytometer Instruction Manual, serial number 2400 and higher; BD Accuri™ C6 Software User Guide; BD CSampler™ Accessory Installation Guide; BD CSampler™ Software User Guide Accuri C6 is an "easy to use" flow cytometer which can be connected to an autosampler (48 and 96 well, and 24 tube rack, we do not have one). Accuri C6 consumables, manuals, etc. Includes all accessories shown in pictures. This includes microfuge tubes and tubes made of polystyrene and polypropylene. This manual provides information on cytometer requirements and specifications, setup, and maintenance. The unique fluidics system of the BD Accuri C6 Plus, driven by peristaltic pumps, allows it to determine sample volume and count cells rapidly, directly, and automatically from the software, eliminating laborious plate counts. BD Accuri C6 Software provides the following features: a. Tabbed views for collection, analysis, and statistics b. Instructional video on the basics of using BYU's Accuri C6 cytometer. h�b```d``ja`c``�� Ā B,@Q�G����^�M�*O�:.:�c�a��"~��!������Q���ȵ�۵8�1}p�l�����C�+x��L��a:_"�����.��r+��枘�! The BD Accrui C6 Cytometer -The cytometer has 2 lasers: -Blue 488nm laser (3 colors) -Red 640 (1 color) -Unlike most cytometers you will encounter, the Accuri does not all you to manually adjust your voltage settings -Instead, the software uses a larger logarithmic … A broad dynamic range of detection makes it easy to analyze cells as varied in size as platelets and eosinophils in the same data file. For Research Use Only. With minor signs of normal wear and tear. x������ PP9R�P������t�Y�a9D��� ` +9|%��; ��0^��O���-�&+�M�n����I��]�: ���bh��*��f��M�=�>�����}'$�� ���r��n�Ypm8I_�À�W�"v����,,{�m��c__$�`�r݀��\NiC�{i\ �p=�ƫ�k.x���8�ႃ3��{a�> �6111111111111111��Ǧ�� If you are interested in using a fluorochrome that is not listed here with the Accuri C6, please contact the operator.. Click here to view the instrument manual. We have 1 BD Accuri C6 manual available for free PDF download: Instrument Manual . The software for Accuri C6 (CFlow Plus) is very user-friendly and efficient. User's Guides and Manuals. Developed the C6 fluidics system is non-pressurized, peristaltic pump system drives fluidics... View with FCS Express Import Demo.c6. filters can be used is FCS Express Import Demo.c6. peristaltic pump drives. Update Driver Software - cytometer, Second Dialog Box, Figure 5-4 drives the fluidics fluidics system is non-pressurized any... 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